Composite

Part:BBa_J70638:Design

Designed by: Julie Norville   Group: Knight Lab   (2010-11-18)

rbs VioA rbs VioB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1029
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3814
    Illegal AgeI site found at 4010
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

there was a sequence between the stop codon of vioB and rbs of vioC in original part


Source

derived from K274002

J70638 A Prefix: GCGGCCGCTTCTAGAGTTAAGGAGGTAAAAAAAATGAAACATTCTTCC

J70638 B Suffix: GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGATCCTTAGGCCTCGCGGCTCAG

References